Staining by anti-Ep-CAM / CD326 Antibody 1

Anti-Epcam / CD326 Antibody Clone SPM491

$ 298.00
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SKU: 4072-MSM11X
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 4072-MSM11X-P1
Epcam / CD326 General Information
Alternate Names
EpCAM, Epithelial cell adhesion molecule, EGP40, CD326, Cluster of Differentiation 326
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolEPCAM
Entrez Gene ID4072
Ensemble Gene IDENSG00000119888
RefSeq Protein Accession(s)NP_002345
RefSeq mRNA Accession(s)NM_002354,
RefSeq Genomic Accession(s)NG_012352, NC_000002, NC_018913
UniProt ID(s)P16422
UniGene ID(s)P16422
HGNC ID(s)11529
Cosmic ID(s)EPCAM
KEGG Gene ID(s)hsa:4072
PharmGKB ID(s)PA35493
General Description of Epcam / CD326.
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Epcam). It is expressed on baso-lateral cell surface in most simple epithelia, a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma, hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Human, Mouse (-), and Rat (-) Anti-Epcam / CD326 Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Epcam / CD326 Antibody Clone: SPM491
Clone SPM491 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse (-), and Rat (-) Epcam / CD326 Positive Control Sample: HT29 cells or Ovarian carcinoma
Cellular Localization of Antibody Cell Surface & Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human EpCAM protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Epcam / CD326 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-EPCAM antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Epcam / CD326 expression as measured by anti-Epcam / CD326 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + + +++ +++ - - - +++ - - +++ - +++ - - - - +++ -
EPCAM Variability ++ +++ +++ + + + ++ + ++ + + ++ + + + ++ ++ ++ + +

Limitations and Warranty

enQuire Bio's Epcam / CD326 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Epcam / CD326 Antibody Clone SPM491