Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Monoclonal Antibody (SPM256)
Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Monoclonal Antibody (SPM256)

Anti-Histone H1 Antibody Clone SPM256

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3005-MSM1X
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
3005-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
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SKU: 3005-MSM1X-P1
Histone H1 General Information
Alternate Names
Histone H1
Molecular Weight
Chromosomal Location
3q21-q22; 6q21-q22; 22q13.1
Curated Database and Bioinformatic Data
Gene SymbolH1
Entrez Gene ID3005
Ensemble Gene IDENSG00000189060
RefSeq Protein Accession(s)NP_005309
RefSeq mRNA Accession(s)NM_005318
RefSeq Genomic Accession(s)NC_000022, NC_018933
UniProt ID(s)P07305
UniGene ID(s)P07305
HGNC ID(s)4714
Cosmic ID(s)H1F0
KEGG Gene ID(s)hsa:3005
PharmGKB ID(s)PA29092
General Description of Histone H1.
Eukaryotic histones are basic, water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3,, H4) form the octamer; formed of two H2A-H2B dimers, two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes, mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination, ADP-ribosylation.

Human, Mouse, and Rat Anti-Histone H1 Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Histone H1 Antibody Clone: SPM256
Clone SPM256 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Mouse, and Rat Histone H1 Positive Control Sample: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Nuclei of human leukemia biopsy cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Histone H1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-H1F0 antibody staining is expected to be primarily localized to the nuclear bodies and nucleus. There is variability in either the signal strength or the localization of signal in nucleus from cell to cell.

Limitations and Warranty

enQuire Bio's Histone H1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Histone H1 Antibody Clone SPM256