Formalin-fixed, paraffin-embedded Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (MGN185)
Formalin-fixed, paraffin-embedded Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (MGN185)

Anti-Myogenin Antibody Clone MGN185

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4656-MSM1
Species: Human, Mouse, Rat, Cat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4656-MSM1-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 4656-MSM1-P1
Myogenin General Information
Alternate Names
Myogenin, myogenic factor 4, MYOG
Molecular Weight
34kDa
Chromosomal Location
1q32.1
Curated Database and Bioinformatic Data
Gene SymbolMYOG
Entrez Gene ID4656
Ensemble Gene IDENSG00000122180
RefSeq Protein Accession(s)NP_002470
RefSeq mRNA Accession(s)NM_002479
RefSeq Genomic Accession(s)NC_000001, NC_018912
UniProt ID(s)P15173
UniGene ID(s)P15173
HGNC ID(s)7612
Cosmic ID(s)MYOG
KEGG Gene ID(s)hsa:4656
PharmGKB ID(s)PA31417
General Description of Myogenin.
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5,, MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle, play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length, includes both a basic DNA binding motif, a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue,, is expressed in tumor cell nuclei of rhabdomyosarcoma, some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Human, Mouse, Rat, Cat, and Pig Anti-Myogenin Antibody Product Attributes

Species: Human, Mouse, Rat, Cat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Myogenin Antibody Clone: MGN185
Clone MGN185 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, Rat, Cat, and Pig Myogenin Positive Control Sample: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human myogenin recombinant protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Myogenin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MYOG antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Myogenin expression as measured by anti-Myogenin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + - - - + + + - + + ++ - + + - - + - -
MYOG Variability ++ ++ ++ + ++ ++ ++ ++ + ++ ++ ++ ++ ++ +++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Myogenin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Myogenin Antibody Clone MGN185

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