Human, Mouse, Rat, Cat, and Pig Anti-Myogenin Antibody Product Attributes
Species: Human, Mouse, Rat, Cat, and Pig
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Myogenin Antibody Clone: MGN185 + F5D
Clone MGN185 + F5D Host and Isotype: Mouse IgG
Anti-Human, Mouse, Rat, Cat, and Pig Myogenin Positive Control Sample: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human myogenin recombinant protein (MGN185); Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Myogenin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MYOG antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Myogenin expression as measured by anti-Myogenin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | + | + | - | - | - | + | + | + | - | + | + | ++ | - | + | + | - | - | + | - | - |
MYOG Variability | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's Myogenin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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