Formalin-fixed, paraffin-embedded human Tonsil stained with Transglutaminase II Monoclonal Antibody (SPM592)
Formalin-fixed, paraffin-embedded human Tonsil stained with Transglutaminase II Monoclonal Antibody (SPM592)

Anti-Transglutaminase II Antibody Clone SPM592

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7052-MSM1X
Species: Human, Monkey, Rabbit, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7052-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 7052-MSM1X-P1
Transglutaminase II General Information
Alternate Names
Tissue transglutaminase, tTG, TG2
Molecular Weight
77-85kDa
Chromosomal Location
20q12
Curated Database and Bioinformatic Data
Gene SymbolTGM2
Entrez Gene ID7052
Ensemble Gene IDENSG00000198959
RefSeq Protein Accession(s)NP_001310247, NP_001310246, NP_945189, NP_001310245, XP_011527330, NP_004604
RefSeq mRNA Accession(s)NM_198951, NM_001323316, NM_001323317, NM_004613, XM_011529028, NM_001323318
RefSeq Genomic Accession(s)NC_000020, NC_018931
UniProt ID(s)B4DTN7, P21980, B4DIT7, V9HWG3
UniGene ID(s)B4DTN7, P21980, B4DIT7, V9HWG3
HGNC ID(s)11778
Cosmic ID(s)TGM2
KEGG Gene ID(s)hsa:7052
PharmGKB ID(s)PA36491
General Description of Transglutaminase II.
Recognizes a 77-85kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites, their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid,, appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation,, wound healing.

Human, Monkey, Rabbit, Mouse, and Rat Anti-Transglutaminase II Antibody Product Attributes

Species: Human, Monkey, Rabbit, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml, 30 min at RT)
Clonality: Monoclonal
Anti-Transglutaminase II Antibody Clone: SPM592
Clone SPM592 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Monkey, Rabbit, Mouse, and Rat Transglutaminase II Positive Control Sample: HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human TGM2 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Transglutaminase II Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TGM2 antibody staining is expected to be primarily localized to the cytosol and plasma membrane. There is variability in either the signal strength or the localization of signal in cytosol and plasma membrane from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Transglutaminase II antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in decidual cells in the placenta and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in decidual cells in the placenta and trophoblastic cells in the placenta. Low, but measureable presence of Transglutaminase II could be seen inlymphoid tissue in appendix, endothelial cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, melanocytes in skin, adipocytes in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis and urothelial cells in the urinary bladder. We were unable to detect Transglutaminase II in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Transglutaminase II Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Transglutaminase II Antibody Clone SPM592

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