Human, Monkey, Rabbit, Mouse, and Rat Anti-Transglutaminase II Antibody Product Attributes
Species: Human, Monkey, Rabbit, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml, 30 min at RT)
Anti-Transglutaminase II Antibody Clone: TGM2/419
Clone TGM2/419 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Monkey, Rabbit, Mouse, and Rat Transglutaminase II Positive Control Sample: HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human TGM2 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Transglutaminase II Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-TGM2 antibody staining is expected to be primarily localized to the cytosol and plasma membrane. There is variability in either the signal strength or the localization of signal in cytosol and plasma membrane from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in Transglutaminase II antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in decidual cells in the placenta and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in decidual cells in the placenta and trophoblastic cells in the placenta. Low, but measureable presence of Transglutaminase II could be seen inlymphoid tissue in appendix, endothelial cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, melanocytes in skin, adipocytes in mesenchymal tissue, glandular cells in the stomach, Leydig cells in the testis and urothelial cells in the urinary bladder. We were unable to detect Transglutaminase II in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Transglutaminase II Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.