Analysis of Mass Spec data (dashed-line) of fractions stained with Vimentin MS-QAVA™ monoclonal antibody [Clone: VM1170] (solid-line), reveals that less than 4.5% of signal is attributable to non-specific binding of anti-Vimentin [Clone VM1170] to targets other than VIM protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.

Anti-Vimentin Antibody Clone VM1170

$ 298.00
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SKU: 7431-MSM3
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: MS-QAVA™ Validated

Product NumberDescriptionPrice
7431-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7431-MSM3-P1
Vimentin General Information
Alternate Names
Vimentin, Anti-Vimentin
Molecular Weight
57-60kDa
Chromosomal Location
10p12.33
Curated Database and Bioinformatic Data
Gene SymbolVIM
Entrez Gene ID7431
Ensemble Gene IDENSG00000026025
RefSeq Protein Accession(s)NP_003371, XP_006717563
RefSeq mRNA Accession(s)XM_006717500, NM_003380
RefSeq Genomic Accession(s)NC_018921, NC_000010, NG_012413
UniProt ID(s)P08670, V9HWE1
UniGene ID(s)P08670, V9HWE1
HGNC ID(s)12692
Cosmic ID(s)VIM
KEGG Gene ID(s)hsa:7431
PharmGKB ID(s)PA37311
General Description of Vimentin.
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament,, glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas, large cell lymphomas. All melanomas, Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells,, fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma, seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Human, Mouse (-), and Rat (-) Anti-Vimentin Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Vimentin Antibody Clone: VM1170
Clone VM1170 Host and Isotype: Mouse IgG1
Anti-Human, Mouse (-), and Rat (-) Vimentin Positive Control Sample: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human vimentin protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Vimentin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Vimentin Anti-Human antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, endothelial cells in the cerebral cortex and colon, glandular cells in the epididymis and fallopian tube, cells in the glomeruli in kidney, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the seminal vesicle, fibroblasts in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, endothelial cells in the cerebral cortex and colon, glandular cells in the epididymis and fallopian tube, cells in the glomeruli in kidney, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the seminal vesicle, fibroblasts in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of Vimentin Anti-Human could be seen inglandular cells in the adrenal gland, respiratory epithelial cells in the bronchus, glial cells in the cerebral cortex, cells in the endometrial stroma in endometrium, glial cells in the hippocampus, bile duct cells in the liver, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, decidual cells in the placenta and squamous epithelial cells in the vagina. We were unable to detect Vimentin Anti-Human in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Vimentin Anti-Human expression as measured by anti-Vimentin Anti-Human antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - + - ++ - ++ ++ ++ +++ +++ + ++ - +++ ++ ++ ++ +++ ++
VIM Variability + + ++ ++ ++ ++ ++ ++ + ++ + ++ + ++ + ++ + ++ + ++

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Vimentin Antibody Clone VM1170

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