SDS-PAGE Analysis Purified p53 Recombinant Rabbit Monoclonal Antibody (TP53/2092R).Confirmation of Purity and Integrity.
SDS-PAGE Analysis Purified p53 Recombinant Rabbit Monoclonal Antibody (TP53/2092R).Confirmation of Purity and Integrity.Analysis of Protein Array containing more than 19,000 full-length human proteins using p53 Recombinant Rabbit Monoclonal Antibody (TP53/2092R).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Western blot analysis of Hela cell lysate using p53 Recombinant Rabbit Monoclonal Antibody (TP53/2092R).

p53 Tumor Suppressor Protein Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 159.00$ 349.00
Please Select A Product To View The SKU.

SKU: 7157-RBM17
Species: Human, Monkey, Dog, Hamster and Chicken. Does not react with mouse and rat.
Tested Applications: Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Rabbit IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 7157-RBM17-P0
p53 Tumor Suppressor Protein General Information
Alternate Names
Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53
Molecular Weight
53kDa
Chromosomal Location
Ships on blue ice.
Curated Database and Bioinformatic Data
Gene Symbol7157
Entrez Gene IDTP53
UniProt ID(s)P04637
UniGene ID(s)Hs654481
COSMIC ID Link(s)TP53
KEGG Gene ID(s)hsa:7157
General Description of p53 Tumor Suppressor Protein.
This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Human, Monkey, Dog, Hamster and Chicken. Does not react with mouse and rat. Anti-p53 Tumor Suppressor Protein Antibody Product Attributes

Species: Human, Monkey, Dog, Hamster and Chicken. Does not react with mouse and rat.
Tested Applications: Flow, IF, WB, IHC.
Clonality: Monoclonal
Anti-p53 Tumor Suppressor Protein Antibody Clone: TP53/2092R
Clone TP53/2092R Host and Isotype: Rabbit IgG
Anti-Human, Monkey, Dog, Hamster and Chicken. Does not react with mouse and rat. p53 Tumor Suppressor Protein Positive Control Sample: MDA-MB-231 Cells. Breast or Colon carcinoma
Cellular Localization of Antibody TP53/2092R Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A column purification.
Immunogen: Recombinant human wild-type p53 protein
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

p53 Tumor Suppressor Protein Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in p53 Tumor Suppressor Protein antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of p53 Tumor Suppressor Protein could be seen inglandular cells in the adrenal gland, hematopoietic cells in the bone marrow, glandular cells in the breast, respiratory epithelial cells in the bronchus, neuronal cells in the caudate nucleus, glandular cells in the cervix, uterine and endometrium, cells in the glomeruli in kidney, islets of Langerhans in pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, glandular cells in the salivary gland, fibroblasts in skin, cells in the white pulp in spleen, non-germinal center cells in the tonsil and squamous epithelial cells in the vagina. We were unable to detect p53 Tumor Suppressor Protein in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of p53 Tumor Suppressor Protein expression as measured by anti-p53 Tumor Suppressor Protein antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - + + - - - - + - + - ++ - - + + + - ++
TP53 Variability + + ++ ++ + ++ ++ ++ ++ ++ +++ ++ ++ + + ++ ++ ++ + +++

Limitations and Warranty

enQuire Bio's product, p53 Tumor Suppressor Protein MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.

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Tested against >20,000 Human Proteins

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p53 Tumor Suppressor Protein Ultraspecific Antibody

Tested against >20,000 Human Proteins

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