Human TNF-alpha Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TNF-alpha based upon sequence from: Human
Host: QP10353 protein expressed in E. coli.
Protein Construction: A DNA sequence encoding the Human TNF-alpha, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10353.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 Ã 108 IU/mg in the presence of actinomycin D.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Concentration of Human TNF-alpha Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 m filtered 20 mM PBS, 10 mM Nacl, pH 7.0, lyophilized
Storage Conditions: Store at -20C to -80C.
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.