C57Bl/6 splenocytes were stained with 0.5 ug APC Mouse Anti-CD3 (QAB3) (solid line) or 0.5 ug APC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD3 Antibody [17A2]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;PE ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB3-F-25ug
CD3 General Information
Curated Database and Bioinformatic Data
Gene SymbolCd3g
Entrez Gene ID12502
Ensemble Gene IDENSMUSG00000002033
RefSeq Protein Accession(s)NP_033980
RefSeq mRNA Accession(s)NM_009850
RefSeq Genomic Accession(s)NC_000075
UniProt ID(s)P11942, Q3U4Y3
UniGene ID(s)P11942, Q3U4Y3
Cosmic ID(s)Cd3g
KEGG Gene ID(s)mmu:12502
General Description of CD3.
The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. Journal of Biological Chemistry. 287: 13324-13335).

Mouse Anti-CD3 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: 17A2
Clone 17A2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD3 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen and non-germinal center cells in the tonsil. Low, but measureable presence of CD3 could be seen inlymphoid tissue in appendix. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD3 expression as measured by anti-CD3 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - + - - - - - - - - - - - - - ++ -
CD3G Variability + ++ + ++ ++ + ++ + + + + + ++ ++ + + + + ++ +

Selected References

Joetham A, Ohnishi H, Okamoto M, Takeda K, Schedel M, Domenico J, Dakhama A, and Gelfand EW. 2012. J. Biol. Chem. 287:17100-17108. (in vitro activation)Kasahara S and Clark, EA. 2012. J. Leukoc. Biol. 91:437-448. (in vitro activation)Xiao J, Julianty A, Wen J, Smith SV, Park PW, Ford ML, Haller CA, and Chaikof EL. 2012. Arterioscler. Thromb. Vasc. Biol. 32:386-396. (in vivo T cell depletion)Bas A, Swamy M, Abeler-Dorner L, Williams G, Pang DJ, Barbee SD, and Hayday AC. 2011 Proc. Natl. Acad. Sci. 108: 4376-4381. (Immunohistochemistry - Paraffin embedded sections)Miescher GC, Schreyer M, and MacDonald HR. 1989. Immunol. Lett. 23: 113-118. (Origination of clone 17A2, Functional Assay, Immunohistochemistry, Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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