Mouse Anti-CD3 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD3 Antibody Clone: 17A2
Clone 17A2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD3 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen and non-germinal center cells in the tonsil. Low, but measureable presence of CD3 could be seen inlymphoid tissue in appendix. We were unable to detect CD3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD3 expression as measured by anti-CD3 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Joetham A, Ohnishi H, Okamoto M, Takeda K, Schedel M, Domenico J, Dakhama A, and Gelfand EW. 2012. J. Biol. Chem. 287:17100-17108. (in vitro activation)Kasahara S and Clark, EA. 2012. J. Leukoc. Biol. 91:437-448. (in vitro activation)Xiao J, Julianty A, Wen J, Smith SV, Park PW, Ford ML, Haller CA, and Chaikof EL. 2012. Arterioscler. Thromb. Vasc. Biol. 32:386-396. (in vivo T cell depletion)Bas A, Swamy M, Abeler-Dorner L, Williams G, Pang DJ, Barbee SD, and Hayday AC. 2011 Proc. Natl. Acad. Sci. 108: 4376-4381. (Immunohistochemistry - Paraffin embedded sections)Miescher GC, Schreyer M, and MacDonald HR. 1989. Immunol. Lett. 23: 113-118. (Origination of clone 17A2, Functional Assay, Immunohistochemistry, Immunoprecipitation)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.