PDF DatasheetHuman P4HB / ERBA2L Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant P4HB / ERBA2L based upon sequence from: Human
Host: QP10155 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Human P4HB / ERBA2L, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10155.
Bioactivity Data: Thiol Protein Reductase Activity is 0.001 delta650nm/ min-2, determined by measuring the turbidity increase at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MRGSGSHHHH HHAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human P4HB / ERBA2L Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.0, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human P4HB / ERBA2L Protein General Information | |
|---|---|
| Alternate Names | |
| P4HB; ERBA2L; PHDB; P4Hbeta; PO4DB; PROHB; ERBA2L; GIT; DSI; PDI; CLCRP1; PDIA1; PO4HB | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | P4HB |
| Entrez Gene ID | 5034 |
| Ensemble Gene ID | ENSG00000185624 |
| RefSeq Protein Accession(s) | NP_000909.2 |
| RefSeq mRNA Accession(s) | NM_000918.3 |
| UniProt ID(s) | P07237 |
| UniGene ID(s) | Hs.464336 |
| HGNC ID(s) | HGNC:8548 |
| COSMIC ID Link(s) | P4hb |
| KEGG Gene ID(s) | hsa:5034 |
| PharmGKB ID(s) | PA32876 |
| General Description of Recombinant Human P4HB / ERBA2L Protein. | |
| This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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