Flow Cytometry of human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: Alexa Fluor® 488-labeled CD31 Monoclonal Antibody (C31.7).
Flow Cytometry of human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: Alexa Fluor® 488-labeled CD31 Monoclonal Antibody (C31.7).

Anti-CD31 / PECAM-1 Antibody Clone C31.7

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5175-MSM1
Species: Human, Cynomolgus Monkey, Rabbit, and Rat (Weak)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5175-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 5175-MSM1-P1
CD31 / PECAM-1 General Information
Alternate Names
Platelet endothelial cell adhesion molecule, PECAM-1, cluster of differentiation 31, CD31, PECAM
Molecular Weight
~100kDa (endothelium), ~130kDa (platelets)
Chromosomal Location
17q23.3
Curated Database and Bioinformatic Data
Gene SymbolPECAM1
Entrez Gene ID5175
Ensemble Gene IDENSG00000261371
RefSeq Protein Accession(s)XP_005276937, XP_016880229, NP_000433, XP_016880227, XP_016880228, XP_011523191, XP_005276938, XP_011523192, XP_005276939, XP_016880230, XP_005276940
RefSeq mRNA Accession(s)XM_011524890, XM_017024739, NM_000442, XM_005276881, XM_017024738, XM_005276880, XM_005276882, XM_011524889, XM_005276883, XM_017024740
RefSeq Genomic Accession(s)NG_047009, NC_000017, NC_018928
UniProt ID(s)P16284, A0A075B738
UniGene ID(s)P16284, A0A075B738
HGNC ID(s)8823
Cosmic ID(s)PECAM1
KEGG Gene ID(s)hsa:5175
PharmGKB ID(s)PA33167
General Description of CD31 / PECAM-1.
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system, is primarily used to identify, concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific, sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign,, malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis,, a high level of CD31 expression may imply a rapidly growing tumor, potentially a predictor of tumor recurrence.

Human, Cynomolgus Monkey, Rabbit, and Rat (Weak) Anti-CD31 / PECAM-1 Antibody Product Attributes

Species: Human, Cynomolgus Monkey, Rabbit, and Rat (Weak)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD31 / PECAM-1 Antibody Clone: C31.7
Clone C31.7 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Cynomolgus Monkey, Rabbit, and Rat (Weak) CD31 / PECAM-1 Positive Control Sample: Jurkat cells. Tonsil or Angiosarcoma.
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human recombinant CD31 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD31 / PECAM-1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD31 / PECAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. More moderate antibody staining intensity was present in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. Low, but measureable presence of CD31 / PECAM-1 could be seen inmacrophages in lung. We were unable to detect CD31 / PECAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's CD31 / PECAM-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD31 / PECAM-1 Antibody Clone C31.7

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