Human Anti-CD31 / PECAM-1 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Anti-CD31 / PECAM-1 Antibody Clone: SPM532
Clone SPM532 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD31 / PECAM-1 Positive Control Sample: Tonsil, Angiosarcoma
Cellular Localization of Antibody Cell surface, cytoplasm of endothelial cells
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human recombinant CD31 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD31 / PECAM-1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD31 / PECAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. More moderate antibody staining intensity was present in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. Low, but measureable presence of CD31 / PECAM-1 could be seen inmacrophages in lung. We were unable to detect CD31 / PECAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's CD31 / PECAM-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.