Human PBMCs were stained with CD19 FITC and 5 uL  (right panel) or 0.125 ug APC Mouse IgG2a isotype control (left panel).

Human Anti-CD5 Antibody [L17F12]

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Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PerCP ;PerCP-Cy5.5
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB14-F-25Tests
CD5 General Information
Alternate Names
CD5, LEU1, Ly-1, Ly-12, Ly-A, Lyt-1
Curated Database and Bioinformatic Data
Gene SymbolCD5
Entrez Gene ID921
Ensemble Gene IDENSG00000110448
RefSeq Protein Accession(s)NP_001333385, NP_055022
RefSeq mRNA Accession(s)NM_014207, NM_001346456
RefSeq Genomic Accession(s)NC_018922, NC_000011,
UniProt ID(s)P06127
UniGene ID(s)P06127
HGNC ID(s)1685
Cosmic ID(s)CD5
KEGG Gene ID(s)hsa:921
PharmGKB ID(s)PA26224
General Description of CD5.
The L17F12 antibody is specific for human CD5, a 67 kD transmembrane glycoprotein that is expressed on most thymocytes, mature T cells, and a subset of B cells. CD5 is a member of the scavenger receptor superfamily and is present on approximately 70% of normal peripheral blood lymphocytes. CD5 is involved in modulating antigen receptor signaling in both T and B cells and acts through binding of CD72, its receptor expressed on B cells.

Human Anti-CD5 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: L17F12
Clone L17F12 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.Gadol N and Ault KA. 1986. Immunol Rev. 93: 23-34.Antin JH, Emerson SG, Martin P, Gadol N and Ault KA. 1986. J Immunol. 136: 505-510.Raab M, Yamamoto M and Rudd CE. 1994. Mol Cell Biol. 14(5): 2862-2870.Lydyard PM, Lamour A, MacKenzie LE, Jamin C, Magged RA and Youinou P. 1993. Immunol Lett. 38(2): 159-166.McKenna RW, Washington LT, Aquino DB, Picker LJ and Kroft SH. 2001. Blood. 98(8): 2498-2507. (Flow Cytometry)Butmarc JR, Kourea HP, Levi E and Kadin ME. 1998. Am J Clin Path. 109(6): 682-688. (Immunohistochemistry - paraffin-embedded tissue)

Limitations and Warranty

enQuire Bio's Human Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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