PDF DatasheetMouse CD40L / CD154 / TNFSF5 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CD40L / CD154 / TNFSF5 based upon sequence from: Mouse
Host: QP5411 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5411.
Bioactivity Data: Recombinant Mouse CD40L / CD154 / TNFSF5 induced IL-8 production in human PBMCs detected via ELISA (typical ED50 is 1-10 ng/ml). Activity is also measured in HEK-Blue&trade. CD40L validation criteria in this assay is an ED50 of less than 50 ng/ml.
Monomer or Dimer: Monomer
Amino Acid Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Mouse CD40L / CD154 / TNFSF5 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium phosphate, 50 mM arginine, pH7.5
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Mouse CD40L / CD154 / TNFSF5 Protein General Information | |
|---|---|
| Alternate Names | |
| CD40L, CD154, TNFSF5, CD154, IGM, Ly-62, TRAP, Tnlg8b, HIGM1, CD40-L, Cd40l, Ly62, T-BAM, IMD3, Tnfsf5, gp39 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD40LG |
| Entrez Gene ID | 21947 |
| Ensemble Gene ID | ENSMUSG00000031132 |
| RefSeq Protein Accession(s) | NP_035746.2 |
| RefSeq mRNA Accession(s) | NM_011616.2 |
| UniProt ID(s) | P27548 |
| UniGene ID(s) | Mm.4861 |
| COSMIC ID Link(s) | CD40LG |
| KEGG Gene ID(s) | mmu:21947 |
| General Description of Recombinant Mouse CD40L / CD154 / TNFSF5 Protein . | |
| CD40 Ligand (CD40L), or CD154, is a membrane glycoprotein and differentiation antigen that is expressed on the surface of T cells, monocytes, basophils, eosinophils, platelets, dendritic cells, and endothelial cells. CD40L binds and activates the CD40 receptor on antigen-presenting cells. CD40L stimulates B cell proliferation, immunoglobulin class switching, and antibody secretion. CD40L also induces cytokine production and tumoricidal activity in peripheral blood monocytes. CD40L is a co-stimulator of activated T cell proliferation and inflammatory protein production. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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