PDF DatasheetGilthead Seabream GH Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GH based upon sequence from Gilthead Seabream
Host: QP10633 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth-Hormone Gilthead Seabream in 0.4% NaHCO3 or water adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Bioactivity Data: Binding assays of the 125I-labeled gilthead seabream GH to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant gilthead seabream Growth Hormone, like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to Sparus aurata larvae or intraperitoneally to juvenile fish.
Amino Acid Sequence: MDRVVLMLSV MSLGVSSQPI TDGQRLFSIA VSRVQHLHLL AQRLFSDFES SLQTEEQRQL NKIFLQDFCN SDYIISPIDK HETQRSSVLK LLSISYRLVE SWEFPSRSLS GGSAPRNQIS PKLSELKTGI HLLIRANEDG AEIFPDSSAL QLAPYGNYYQ SLGTDESLRR TYELLACFKK DMHKVETYLT VAKCRLSPEA NCTL
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.02% NaHCO3.
Storage Conditions: Lyophilized Growth-Hormone Gilthead Seabream although stable at room temperature for at least two weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH can be stored at 4°C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Gilthead Seabream GH General Information | |
|---|---|
| Alternate Names | |
| GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin. | |
| Molecular Weight | |
| 21.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P29971 |
| General Description of Recombinant Gilthead Seabream GH. | |
| Somatotropin Gilthead Seabream Recombinant Sparus Aurata produced in E. Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids with an additional Ala at the N-terminus and having a molecular mass of 21.4 kDa. The Gilthead Seabream Growth-Hormone Recombinant is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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