PDF DatasheetGilthead Sea Bream GH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GH based upon sequence from Gilthead Sea Bream
Host: QP11961 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the Sea Bream sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth Hormone Sea Bream in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MDRVVLMLSV MSLGVSSQPI TDGQRLFSIA VSRVQHLHLL AQRLFSDFES SLQTEEQRQL NKIFLQDFCN SDYIISPIDK HETQRSSVLK LLSISYRLVE SWEFPSRSLS GGSAPRNQIS PKLSELKTGI HLLIRANEDG AEIFPDSSAL QLAPYGNYYQ SLGTDESLRR TYELLACFKK DMHKVETYLT VAKCRLSPEA NCTL
Purity: Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3.
Storage Conditions: Lyophilized Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sea Bream GH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Gilthead Sea Bream GH General Information | |
|---|---|
| Alternate Names | |
| GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin. | |
| Molecular Weight | |
| 21.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P29971 |
| General Description of Recombinant Sea Bream GH. | |
| Recombinant GH produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21810 Dalton. GH is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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