PDF DatasheetPorcine GH Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GH based upon sequence from Porcine
Host: QP10637 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized pST in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Somatotropin contains 3 units/mg.
Amino Acid Sequence: MAAGPRTSAL LAFALLCLPW TREVGAFPAM PLSSLFANAV LRAQHLHQLA ADTYKEFERA YIPEGQRYSI QNAQAAFCFS ETIPAPTGKD EAQQRSDVEL LRFSLLLIQS WLGPVQFLSR VFTNSLVFGT SDRVYEKLKD LEEGIQALMR ELEDGSPRAG QILKQTYDKF DTNLRSDDAL LKNYGLLSCF KKDLHKAETY LRVMKCRRFV ESSCAF
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Growth-hormone (1 mg/ml) was lyophilized after extensive dialyses against 0.34 mg sodium phosphate buffer (0.02 mg sodium phosphate monobasic & 0.32 mg sodium phosphate dibasic).
Storage Conditions: Lyophilized GH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Somatotropin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Porcine GH General Information | |
|---|---|
| Alternate Names | |
| GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin, pST. | |
| Molecular Weight | |
| 21.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P01248 |
| General Description of Recombinant Porcine GH. | |
| Porcine-Somatotropin Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21730 Dalton. Growth Hormone is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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