PDF DatasheetChicken GH Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GH based upon sequence from Chicken
Host: QP10632 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized GH Chicken Recombinant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Bioactivity Data: GH Chicken Recombinantis fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.
Amino Acid Sequence: MAPGSWFSPL LIAVVTLGLP QEAAATFPAM PLSNLFANAV LRAQHLHLLA AETYKEFERT YIPEDQRYTN KNSQAAFCYS ETIPAPTGKD DAQQKSDMEL LRFSLVLIQS WLTPVQYLSK VFTNNLVFGT SDRVFEKLKD LEEGIQALMR ELEDRSPRGP QLLRPTYDKF DIHLRNEDAL LKNYGLLSCF KKDLHKVETY LKVMKCRRFG ESNCTI
Purity: Greater than 99.0% as determined by:(a) Analysis by SDS-PAGE gel. (b) Analysis by SEC-HPLC.
Concentration of Chicken GH Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Storage Conditions: Lyophilized GH Chicken although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 mg/ml and filter sterilization GH can be stored at 4°C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Chicken GH General Information | |
|---|---|
| Alternate Names | |
| GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin. | |
| Molecular Weight | |
| 22.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P08998 |
| General Description of Recombinant Chicken GH. | |
| Growth Hormone Chicken Recombinant (cGH) produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton. GH Chicken recombinant is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.