PDF DatasheetCarp GH Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GH based upon sequence from Carp
Host: QP10631 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth Hormone Carp recombinant in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Carp GH is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent in vivo in promoting carp growth (Fine et al. 1993). Furthermore, carp GH forms 1:2 complex with the extra cellular domain of ovine growth hormone receptor.
Amino Acid Sequence: MARVLVLLSV VLVSLLVNQG RASDNQRLFN NAVIRVQHLH QLAAKMINDF EDSLLPEERR QLSKIFPLSF CNSDYIEAPA GKDETQKSSM LKLLRISFHL IESWEFPSQS LSGTVSNSLT VGNPNQLTEK LADLKMGISV LIQACLDGQP NMDDNDSLPL PFEDFYLTMG ENNLRESFRL LACFKKDMHK VETYLRVANC RRSLDSNCTL
Purity: Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The GH Carp was lyophilized from a concentrated (1 mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Storage Conditions: Lyophilized Growth Hormone Carp recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Hormone Carp should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Carp GH General Information | |
|---|---|
| Alternate Names | |
| GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin. | |
| Molecular Weight | |
| 21.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P10298 |
| General Description of Recombinant Carp GH. | |
| Growth Hormone Carp Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 188 amino acids & having a molecular mass of 21,408 Dalton. Growth Hormone Carp is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
Growth Hormone Promotes Hair Cell Regeneration in the Zebrafish (Danio rerio) Inner Ear following Acoustic Trauma.Publication:Sun H, Lin C-H, Smith ME (2011) Growth Hormone Promotes Hair Cell Regeneration in the Zebrafish (Danio rerio) Inner Ear following Acoustic Trauma. PLoS ONE 6(11): e28372. doi:10.1371/journal.pone.0028372Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0028372
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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