PDF DatasheetRat GH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GH based upon sequence from Rat
Host: QP11964 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth Hormone Rat in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MAADSQTPWL LTFSLLCLLW PQEAGAFPAM PLSSLFANAV LRAQHLHQLA ADTYKEFERA YIPEGQRYSI QNAQAAFCFS ETIPAPTGKE EAQQRTDMEL LRFSLLLIQS WLGPVQFLSR IFTNSLMFGT SDRVYEKLKD LEEGIQALMQ ELEDGSPRIG QILKQTYDKF DANMRSDDAL LKNYGLLSCF KKDLHKAETY LRVMKCRRFA ESSCAF
Purity: Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein (1 mg/ml) was lyophilized after extensive dialyses against 5mM phosphate buffer, 5 mg mannitol and 1 mg glycine.
Storage Conditions: Lyophilized Growth Hormone Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GH Rat should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat GH General Information | |
|---|---|
| Alternate Names | |
| GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin. | |
| Molecular Weight | |
| 21.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P01244 |
| General Description of Recombinant Rat GH. | |
| Rat Growth Hormone produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21810 Dalton. GH is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
GHRELIN HYPORESPONSIVENESS CONTRIBUTES TO AGING-RELATED HYPERINFLAMMATION IN SEPTIC SHOCK.Publication:Ann Surg. Author manuscript; available in PMC 2010 July 1. Published in final edited form as:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2797311/
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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