Human, Mouse, and Rat Anti-ACTH Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunoprecipitation (0.5-1 ug of antibody/500ug protein Lysate), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Anti-ACTH Antibody Clone: AH26 + 57
Clone AH26 + 57 Host and Isotype: Mouse IgG
Anti-Human, Mouse, and Rat ACTH Positive Control Sample: Normal pituitary gland or pituitary tumor.
Cellular Localization of Antibody Cytoplasm
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH (AH26); N-terminal fragment of human ACTH conjugated to KLH (57)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
ACTH Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in ACTH antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the anterior in pituitary gland. More moderate antibody staining intensity was present in cells in the anterior in pituitary gland. Low, but measureable presence of ACTH could be seen in. We were unable to detect ACTH in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's ACTH Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.