Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).

Anti-Cytokeratin Antibody Clone 34BE12

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: MSM3-446
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: MSM3-446-P1
Cytokeratin General Information
Alternate Names
KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9, anti-KRT2B antibody, anti-KRT2P antibody, anti-HUMCYT2A antibody, anti-Keratin antibody, anti-type II Cytoskeletal 2 oral antibody, anti-K76 antibody, anti-Keratin 2p (K2P) antibody, anti-Keratin-76 antibody, anti-Cytokeratin-2P (CK-2P antibody, anti-Type-II Keratin Kb9 antibody
Molecular Weight
50-67kDa
Chromosomal Location
12q13.13
Curated Database and Bioinformatic Data
Gene SymbolKRT76
Entrez Gene ID51350
Ensemble Gene IDENSG00000185069
RefSeq Protein Accession(s)NP_056932
RefSeq mRNA Accession(s)NM_015848,
RefSeq Genomic Accession(s)NG_012420, NC_000012, NC_018923
UniProt ID(s)Q01546
UniGene ID(s)Q01546
HGNC ID(s)24430
Cosmic ID(s)KRT76
KEGG Gene ID(s)hsa:51350
PharmGKB ID(s)PA147357785
General Description of Cytokeratin.
This antibody recognizes CK1, CK5, CK10, CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells, basal cells in the prostate gland, bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors,, neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells, has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Human, Mouse, and Rat Anti-Cytokeratin Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: 34BE12
Clone 34BE12 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat Cytokeratin Positive Control Sample: PC12 cells, Skin, Prostate carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Solubilized keratin extract from human stratum corneum
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Cytokeratin could be seen in. We were unable to detect Cytokeratin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin expression as measured by anti-Cytokeratin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - - + + - + + - - - + + - - - - - + -
KRT76 Variability ++ ++ ++ ++ ++ + ++ ++ ++ + ++ ++ ++ ++ ++ + ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Cytokeratin, Basic Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-Cytokeratin Antibody Clone 34BE12”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

Anti-Cytokeratin Antibody Clone 34BE12https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-MSM3-446-P1-anti-Cytokeratin-antibody-300x225.jpeg