Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish Anti-TNF-alpha Antibody Product Attributes
Species: Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-paraffin) (2-4ug of antibody/ml for 30 min at RT)
Anti-TNF-alpha Antibody Clone: P/T2
Clone P/T2 Host and Isotype: Mouse IgM kappa
Anti-Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish TNF-alpha Positive Control Sample: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Cellular Localization of Antibody
Cytoplasmic, extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF-?, conjugated to thyroglobulin
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
TNF-alpha Previously Observed Antibody Staining Patterns
Limitations and Warranty
enQuire Bio's TNF-alpha Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.